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| Registros recuperados : 35 | |
| 8. |  | HAMOND, C.; MARTINS, G.; LOUREIRO, A. P.; BREMONT, S.; MEDEIROS, M. A.; BOUHY, P.; LILENBAUM, W. First isolation and characterization of Leptospira interrogans serogroup Australis from swine in Brazil. Pesquisa Veterinária Brasileira, Rio de Janeiro, n. 35, n.1, p.6-8, jan. 2015.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 10. | | SOUZA, G. N.; MOREIRA, E. C.; RISTOW, P.; FRAGUAS, S.; LILENBAUM, W. Formas de exploração do rebanho caprino no Estado do Rio de Janeiro, 1998/2000. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 54, n. 2, p. 221-222, abr. 2002. Comunicacao.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | SOUZA, G. N. de; MOREIRA, E. C.; RISTOW, P.; FRÁGUAS, S.; LILENBAUM, W. Frequência de aglutininas anti-leptospira interrogans em caprinos de aptidão leiteira do Estado do Rio de Janeiro. Revista Brasileira de Medicina Veterinária, Rio de Janeiro, v. 23, n. 6, p. 235-239, 2001.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 13. | | PINNA, A.; MARTINS, G.; HAMOND, C.; MEDEIROS, M. A.; SOUZA, G. N. de; LILENBAUM, W. Potential differences between Leptospira serovars, host-adapted (Bratislava) and incidental (Copenhageni), in determining reproductive disorders in embryo transfer recipient mares in Brazil. Veterinary Record, v. 174, n. 21, article 531, 2014.| Biblioteca(s): Embrapa Gado de Leite. |
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| 14. | | SARTI, E. C. F. B.; RODRIGUES, R. A.; DUARTE, L. F. C.; BACANELLI, G. M.; LILENBAUM, W.; ARAUJO, F. R. Detection of Potentially Pathogenic Non-Tuberculous Mycobacteria in Artisanal Coalho Cheese from the State of Paraiba, Northeast Brazil. Mycobacterial Diseases, v. 8, n. 3, 2018| Biblioteca(s): Embrapa Gado de Corte. |
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| 16. | | COENTRÃO, C. M.; SOUZA, G. N. de; BRITO, J. R. F.; BRITO, M. A. V. P. e; LILENBAUM, W. Fatores de risco para mastite subclínica em vacas leiteiras. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, Belo Horizonte, v. 60, n. 2, p. 283-288, 2008.| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | LILENBAUM, W.; VARGES, R.; MEDEIROS, L.; CORDEIRO, A. G.; CAVALCANTI, A.; SOUZA, G. N. de; RICHTZENHAIN, L.; VASCONCELLOS, S. A. Risk factors associated with leptospirosis in dairy goats under tropical conditions in Brazil. Research in Veterinary Science, Oxford, v. 84, n. 1, p. 14-17, 2008.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| 19. | | OLIVEIRA, J. K.; MARTINS, G.; ESTEVES, L. V.; PENNA, B.; HAMOND, C.; FONSECA, J. F. da; RODRIGUES, A. L.; BRANDÃO, F. Z.; LILENBAUM, W. Changes in the vaginal flora of goats following a short-term protocol of oestrus induction and synchronisation with intravaginal sponges as well as their antimicrobial sensitivity. Small Ruminant Research, Amsterdam, v. 113, n. 1, p. 162-166, June, 2013.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 20. | | RISTOW, P.; RODRIGUES, A. B. F.; FONSECA, L. S.; OELEMANN, W. M. R.; CARVALHO, E. Q.; SOUZA, G. N. de; MARASSI, C. D.; LILENBAUM, W. Correlation between pathological findings and bacteriological culture on paratuberculous cattle. Ciência Animal Brasileira, Goiânia, v. 9, n. 3, p. 700-704, 2008.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 35 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/09/2023 |
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Data da última atualização: |
11/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
AYMÉE, L.; DI AZEVEDO, M. I. N.; REIS, L.; MENDES, J.; CASTRO, F. de D. A. de; CARVALHO-COSTA, F. A. C.; SOUZA, G. N. de; LILENBAUM, W. |
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Afiliação: |
LUIZA AYMÉE, UNIVERSIDADE FEDERAL FLUMINENSE; MARIA ISABEL NOGUEIRA DI AZEVEDO, UNIVERSIDADE FEDERAL FLUMINENSE; LUIZA REIS, UNIVERSIDADE FEDERAL FLUMINENSE; JULIA MENDES, UNIVERSIDADE FEDERAL FLUMINENSE; FÚLVIA DE FÁTIMA ALMEIDA DE CASTRO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; FILIPE ANIBAL CARVALHO-COSTA, INSTITUTO OSWALDO CRUZ; GUILHERME NUNES DE SOUZA, CNPGL; WALTER LILENBAUM, UNIVERSIDADE FEDERAL FLUMINENSE. |
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Título: |
Unconventional sites for diagnosis of leptospirosis in bovine anicteric fetuses. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Animals, v. 13, 2832, 2023. |
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DOI: |
https://doi.org/10.3390/ani13182832 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Bovine leptospirosis is an important reproductive disease and abortion is a major sign, leading to economic impacts. Due to its multifactorial etiology, the proper diagnosis of the cause of the abortion is crucial. Necropsy of the fetuses followed by molecular analysis is recommended for diagnosis, and the investigation mainly occurs in the kidneys and liver. This study aimed to analyze unconventional sites for the presence of leptospiral DNA in bovine anicteric aborted fetuses. Methods: Five fetuses of the same herd were received for necropsy and diagnosis. Conventional lipL32-PCR was performed in the fetuses? kidneys, livers, lungs, hearts, spleens, subcapsular kidney content, abomasal fluid, and in the cavity?s hemorrhagic contents. To complete the investigation, the sera of 30 cows of the herd were collected to perform the serologic screening by Microscopic Agglutination Test. In addition, six subfertile non-pregnant cows from the same herd were selected due to their low reproductive performance, and genital samples (uterine fragment and cervicovaginal mucus) and urine were collected for lipL32-PCR. PCR-positive samples were submitted to a nested PCR of the secY gene and intended for sequencing. Results: The herd presented seroreactive animals (11/30, 36.6%), all against the Sejroe serogroup, with titers between 200 and 1600. In necropsy, four fetuses showed hemorrhagic and anicteric lesions, while one fetus had no macroscopic lesions. Regarding molecular analysis, all the fetuses were positive in lipL32-PCR and the positive sites were the heart, lungs, subcapsular kidney content, thymus, kidneys, liver, and abomasal fluid. Only one fetus presented positive results in the kidney and liver, while three fetuses were positive in the abomasal fluid. Five of six cows were positive for lipL32-PCR, all being positive only in genital samples. Of the fetuses and the cows, seven sequences were obtained and all were identified as Leptospira interrogans serogroup Sejroe serovar Hardjoprajitno. Conclusions: In order to improve the diagnosis of leptospirosis in cows, it is recommended to perform a comprehensive analysis of the samples, beyond the kidneys and liver. Thus, we highly encourage testing multiple organs by PCR to investigate abortions suspected of bovine leptospirosis, particularly in anicteric fetuses. MenosBackground: Bovine leptospirosis is an important reproductive disease and abortion is a major sign, leading to economic impacts. Due to its multifactorial etiology, the proper diagnosis of the cause of the abortion is crucial. Necropsy of the fetuses followed by molecular analysis is recommended for diagnosis, and the investigation mainly occurs in the kidneys and liver. This study aimed to analyze unconventional sites for the presence of leptospiral DNA in bovine anicteric aborted fetuses. Methods: Five fetuses of the same herd were received for necropsy and diagnosis. Conventional lipL32-PCR was performed in the fetuses? kidneys, livers, lungs, hearts, spleens, subcapsular kidney content, abomasal fluid, and in the cavity?s hemorrhagic contents. To complete the investigation, the sera of 30 cows of the herd were collected to perform the serologic screening by Microscopic Agglutination Test. In addition, six subfertile non-pregnant cows from the same herd were selected due to their low reproductive performance, and genital samples (uterine fragment and cervicovaginal mucus) and urine were collected for lipL32-PCR. PCR-positive samples were submitted to a nested PCR of the secY gene and intended for sequencing. Results: The herd presented seroreactive animals (11/30, 36.6%), all against the Sejroe serogroup, with titers between 200 and 1600. In necropsy, four fetuses showed hemorrhagic and anicteric lesions, while one fetus had no macroscopic lesions. Regarding molecular ana... Mostrar Tudo |
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Palavras-Chave: |
Abomasal fluid; Leptospiral infection; LipL32; Molecular analysis. |
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Thesagro: |
Aborto; Bovino; Gado; Leptospirose. |
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Thesaurus NAL: |
Abortion (animals); Cattle; Necropsy. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156556/1/Unconventional-sites-for-diagnosis-of-Leptospirosis-in-bovine-anicteric-fetuses.pdf
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Marc: |
LEADER 03302naa a2200349 a 4500
001 2156556
005 2023-09-11
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/ani13182832$2DOI
100 1 $aAYMÉE, L.
245 $aUnconventional sites for diagnosis of leptospirosis in bovine anicteric fetuses.$h[electronic resource]
260 $c2023
520 $aBackground: Bovine leptospirosis is an important reproductive disease and abortion is a major sign, leading to economic impacts. Due to its multifactorial etiology, the proper diagnosis of the cause of the abortion is crucial. Necropsy of the fetuses followed by molecular analysis is recommended for diagnosis, and the investigation mainly occurs in the kidneys and liver. This study aimed to analyze unconventional sites for the presence of leptospiral DNA in bovine anicteric aborted fetuses. Methods: Five fetuses of the same herd were received for necropsy and diagnosis. Conventional lipL32-PCR was performed in the fetuses? kidneys, livers, lungs, hearts, spleens, subcapsular kidney content, abomasal fluid, and in the cavity?s hemorrhagic contents. To complete the investigation, the sera of 30 cows of the herd were collected to perform the serologic screening by Microscopic Agglutination Test. In addition, six subfertile non-pregnant cows from the same herd were selected due to their low reproductive performance, and genital samples (uterine fragment and cervicovaginal mucus) and urine were collected for lipL32-PCR. PCR-positive samples were submitted to a nested PCR of the secY gene and intended for sequencing. Results: The herd presented seroreactive animals (11/30, 36.6%), all against the Sejroe serogroup, with titers between 200 and 1600. In necropsy, four fetuses showed hemorrhagic and anicteric lesions, while one fetus had no macroscopic lesions. Regarding molecular analysis, all the fetuses were positive in lipL32-PCR and the positive sites were the heart, lungs, subcapsular kidney content, thymus, kidneys, liver, and abomasal fluid. Only one fetus presented positive results in the kidney and liver, while three fetuses were positive in the abomasal fluid. Five of six cows were positive for lipL32-PCR, all being positive only in genital samples. Of the fetuses and the cows, seven sequences were obtained and all were identified as Leptospira interrogans serogroup Sejroe serovar Hardjoprajitno. Conclusions: In order to improve the diagnosis of leptospirosis in cows, it is recommended to perform a comprehensive analysis of the samples, beyond the kidneys and liver. Thus, we highly encourage testing multiple organs by PCR to investigate abortions suspected of bovine leptospirosis, particularly in anicteric fetuses.
650 $aAbortion (animals)
650 $aCattle
650 $aNecropsy
650 $aAborto
650 $aBovino
650 $aGado
650 $aLeptospirose
653 $aAbomasal fluid
653 $aLeptospiral infection
653 $aLipL32
653 $aMolecular analysis
700 1 $aDI AZEVEDO, M. I. N.
700 1 $aREIS, L.
700 1 $aMENDES, J.
700 1 $aCASTRO, F. de D. A. de
700 1 $aCARVALHO-COSTA, F. A. C.
700 1 $aSOUZA, G. N. de
700 1 $aLILENBAUM, W.
773 $tAnimals$gv. 13, 2832, 2023.
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